Monday, November 5, 2007
Saturday, October 20, 2007
BIOGRAFIA
La Dra. Maria Lucrecia Álvarez trabaja en el desarrollo de vacunas en plantas transgénicas para uso terapéutico o industrial. En 1994 se graduó de Bioquímica y en el 2000 obtuvo su Doctorado en Biología Molecular en la Universidad Nacional de Rosario (Argentina). Realizó su tesis doctoral en el tema “Mejoramiento de la Calidad Nutricional y Panadera del trigo por Ingeniería Genética”. Recibió parte de su entrenamiento de doctorado en Inglaterra, en el Institute of Arable Crops Research (IACR), Long Ashton Research Station, Bristol University, Bristol. En el año 2000, la Dra. Álvarez obtuvo una beca de Fundación Antorchas (Argentina) para estudiar las propiedades reológicas de la masa hecha con las harinas provenientes del trigo transgénico que obtuvo. Realizó dichos estudios durante su visita a la Universidad Politécnica de Madrid, Departamento de Genética y Mejoramiento de Plantas (Madrid, España).En el año 2002, la Dra. Alvarez obtuvo una beca de la Fundación Antorchas (Argentina) para trabajar con el grupo de investigación de la Dra. Amanda Walmsley en la Arizona State University, Tempe, Arizona (USA). En el año 2003 fue contratada para hacer sus estudios post-doctorales junto al grupo del Dr. Guy Cardineau (Biodesign Institute, Arizona State University, Tempe, Arizona, Estados Unidos). Actualmente trabaja en el desarrollo de una vacuna oral hecha en tomate transgénico para prevención de peste bubónica y neumónica (Alvarez et. al. 2006, Vaccine 24: 2477- 2490), proyecto subvencionado por el Departamento de Defensa de los Estados Unidos. Otras áreas de su interés incluyen un proyecto de reversión de silenciamiento génico en plantas como estrategia para aumentar la expresión de proteínas recombinantes.
Como parte de un proyecto de colaboración con el Instituto Tecnológico de Monterrey (México), la Dra. Alvarez y el Dr. Cardineau trabajan también en desarrollo de alfalfa transgénica para producir factor estimulante de granulocitos humanos (FEGH). Este factor estimula la formación de glóbulos blancos por lo que seria usado en el tratamiento de pacientes con leucemia y anemia plástica, así como también para contrarrestar los efectos secundarios negativos asociados con la quimioterapia para cáncer.
Friday, October 19, 2007
PUBLICACIONES CIENTIFICAS
Manuscritos en preparación
Pinkhasov J, Alvarez ML, Rigano MM, Mukherjee P, Gendler SJ, Walmsley AM, Mason HS. Expression and immunogenicity of mucosally targeted human epithelial mucin peptide: towards a plant-made breast cancer vaccine. Ver resumen.
Alvarez ML, Martin F, Topal E, Cardineau GA. High accumulation of recombinant proteins in induced ER-derived storage organelles in plants.
Publicaciones
Alvarez ML, Pinyerd HL, Topal E, Cardineau GA. (2008) Transient and stable expression of TBSV-P19 induces reversion of F1-V transgene silencing in tomato. Plant Mol Biol 68: 61-79.
Alvarez ML, Pinyerd HL, Topal E, Cardineau GA. (2008). High f1-v gene expression in transgenic tomato after spontaneous or P19-induced reversion of gene silencing. Proceedings of World Congress on In Vitro Biology. In Vitro Cell Dev. Biol. 44: 56.
Topal E, Alvarez ML, Mason HS. (2008). Plant-derived Intimin Vaccine to Prevent Colonization of Enterohaemorragic Escherichia coli. Proceedings of World Congress on In Vitro Biology. In Vitro Cell Dev. Biol. 44: 36.
Alvarez, M.L, Pinyerd, H., Rigano, M. M., Pinkhasov, J., Walmsley, A.M., Mason, H. S., Cardineau, G. A. (2006). “Plant-made subunit vaccine against pneumonic and bubonic plague is orally immunogenic in mice”. Vaccine 24: 2477- 2490. Ver publicación.
Alvarez ML, Pinyerd HL, Crisantes JD, Rigano MM, Pinkhasov J, Walmsley AM, Mason HS, Cardineau GA (2004). Stable plant expression of F1-V fusion protein antigens from Yersinia pestis for use as a vaccine against plague. In: Proceedings of the REDBIO Congress 2004, Santo Domingo, Dominican Republic. Ver publicación
Rigano, M.M., Alvarez, M.L., Pinkhasov, J., Jin, Y., Sala, F., Arntzen, Ch., Walmsley, A.M. (2004). “Expression and stability of a fusion protein consisting of the enterotoxigenic Escherichia coli heat labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana”. Plant Cell Rep. 22: 502-508. Ver publicación.
Walmsley, A.M., Alvarez, M.L., Jin, Y., Pinkhasov, J., Rigano, M.M., Kirk, D., Mason, H.S., and Arntzen, Ch. (2003). “Expression of an LTB fusion protein in transgenic tomato”. Plant Cell Rep. 21: 1020 – 1026. Ver publicación.
Permingeat, H.G.*, Alvarez, M.L.*, Cervigni, GD, Ravizzini, R. and Vallejos, R.H. (2003). “Stable wheat transformation obtained without selectable markers”. Plant Mol. Biol. 52: 415- 419. (*) Both authors contributed equally with the paper. Ver publicación.
Topal E, Alvarez ML, Mason HS. (2008). Plant-derived Intimin Vaccine to Prevent Colonization of Enterohaemorragic Escherichia coli. Proceedings of World Congress on In Vitro Biology. In Vitro Cell Dev. Biol. 44: 36.
Alvarez, M.L, Pinyerd, H., Rigano, M. M., Pinkhasov, J., Walmsley, A.M., Mason, H. S., Cardineau, G. A. (2006). “Plant-made subunit vaccine against pneumonic and bubonic plague is orally immunogenic in mice”. Vaccine 24: 2477- 2490. Ver publicación.
Alvarez ML, Pinyerd HL, Crisantes JD, Rigano MM, Pinkhasov J, Walmsley AM, Mason HS, Cardineau GA (2004). Stable plant expression of F1-V fusion protein antigens from Yersinia pestis for use as a vaccine against plague. In: Proceedings of the REDBIO Congress 2004, Santo Domingo, Dominican Republic. Ver publicación
Rigano, M.M., Alvarez, M.L., Pinkhasov, J., Jin, Y., Sala, F., Arntzen, Ch., Walmsley, A.M. (2004). “Expression and stability of a fusion protein consisting of the enterotoxigenic Escherichia coli heat labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana”. Plant Cell Rep. 22: 502-508. Ver publicación.
Walmsley, A.M., Alvarez, M.L., Jin, Y., Pinkhasov, J., Rigano, M.M., Kirk, D., Mason, H.S., and Arntzen, Ch. (2003). “Expression of an LTB fusion protein in transgenic tomato”. Plant Cell Rep. 21: 1020 – 1026. Ver publicación.
Permingeat, H.G.*, Alvarez, M.L.*, Cervigni, GD, Ravizzini, R. and Vallejos, R.H. (2003). “Stable wheat transformation obtained without selectable markers”. Plant Mol. Biol. 52: 415- 419. (*) Both authors contributed equally with the paper. Ver publicación.
Alvarez ML, Gómez M, Carrillo JM, Guelman S, Halford N, Lustig S, Reggiardo M, Ryabushkina N, Shewry P, Stein J, Vallejos RH (2001). Transgenic wheat with better breadmaking quality. In: Proceedings of the the 5th National Wheat Congress, Villa Carlos Paz, Argentina. Ver publicación
Alvarez, M.L., Gómez, M., Carrillo, J.M. and Vallejos, R.H. (2001). “Analysis of dough functionality of flour from transgenic wheat”. Mol. Breed. 8: 103-108. Ver publicación.
Alvarez ML, Vallejos RH (2001). El pan nuestro de cada día. Ciencia Hoy, 11: 35-43.Ver publicación
Alvarez, M.L., Gómez, M., Carrillo, J.M. and Vallejos, R.H. (2001). “Analysis of dough functionality of flour from transgenic wheat”. Mol. Breed. 8: 103-108. Ver publicación.
Alvarez ML, Vallejos RH (2001). El pan nuestro de cada día. Ciencia Hoy, 11: 35-43.Ver publicación
Alvarez ML (2000). Tesis Doctoral: Mejoramiento de la calidad nutriocional y panadera del trigo por ingenieria genetica. Facultad de Bioquimica y Farmacia, Universidad Nacional de Rosario, Rosario, Argentina. Ver tesis
Alvarez ML, Guelman S, Halford NG, Lustig S, Reggiardo MI, Ryabushkina N, Shewry P, Stein J, Vallejos RH (2000). Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits. Theor. Appl. Genet.100: 319-327. Ver publicación
Alvarez ML, Laguné A, Jaffré G, Balagué C, Fernández L (1994). Relación entre cepas de enterobacterias manosa resistentes y presencia de células centelleantes en el sedimento urinario. Infect. Microbiol. Clin. 6: 136-140. Ver publicación
Saturday, October 13, 2007
PRESENTACIONES EN CONFERENCIAS NACIONALES E INTERNACIONALES
Alvarez ML, Topal E, Martin F, Cardineau GA (2008). High accumulation of recombinant proteins in induced er-derived storage organelles in plants. Joint Annual Meeting of the American Society of Plant Biologists and the Sociedad Mexicana de Bioquimica, Merida, MEXICO.
Alvarez ML, Pinyerd H, Topal E, Cardineau GA (2008). High f1-v gene expression in transgenic tomato after spontaneous or P19-induced reversion of gene silencing. 2008 World Congress on In vitro Biology, Tucson, Arizona, U.S.A.
Topal E, Alvarez ML, Mason HS. (2008). Plant-derived Intimin Vaccine to Prevent Colonization of Enterohaemorragic Escherichia coli. 2008 World Congress on In vitro Biology, Tucson, Arizona, U.S.A.
Topal E, Alvarez ML, Mason HS. (2008). Plant-derived Intimin Vaccine to Prevent Colonization of Enterohaemorragic Escherichia coli. 2008 World Congress on In vitro Biology, Tucson, Arizona, U.S.A.
Matsuda R, Kubota Ch., Alvarez ML, Gamboa J, Cardineau GA (2008). Biopharmaceutical Production under Controlled Environments: Photosynthetic Rate, Soluble Protein Concentration and Growth of Transgenic Tomato Plants Expressing a Yersinia pestis F1-V Antigen Fusion Protein. 2008 International Meeting on Controlled Environment Agriculture, Cocoa Beach, FL, U.S.A.
Alvarez ML, Pinyerd HL, Topal E, Cardineau GA (2007) Transient and Stable Expression of TBSV-P19 Induces Reversion of f1-v Gene Silencing in Tomato. Valley-wide Biodesign Institute Postdoctoral poster symposium, Tempe, Arizona, U.S.A. Ver resumen en PDF
Rigano MM, Alvarez ML, Pinkhasov J, Izzo AA, Cardi T, Walmsley AM. (2007) Production of an oral, tuberculosis vaccine in transgenic and transplastomic plants. Plant transformation technologies. Vienna, February 4-7, AUSTRIA. Ver poster.
Alvarez ML, Pinyerd HL, Topal E, Ewing M, Crisantes JD, Cardineau GA (2007) Transient and stable expression of TBSV-P19 induces reversion of F1-V gene silencing in tomato. Gene Silencing: the Biology of Small RNAs and the Epigenome. 24th Symposium in Plant Biology. University of California, Riverside, CA, January 18-20, U.S.A. Ver resumen en PDF
Alvarez ML, Pinyerd HL, Crisantes JD, Kilbourne J, Rigano MM, Pinkhasov J, Rocke T, Cardineau GA (2006) Protection against plague in mice using an orally delivered F1-V transgenic tomato vaccine. New Cells for New Vaccines: focus on plant and insect cells technologies, Coral Gables (Miami), FL, September 28-29, U.S.A. Ver resumen en PDF
Topal E, Alvarez ML, O’Brien AD, Mason HS (2006) Plant-made intimin vaccine expressed in tomato for mucosal delivery to prevent colonization of enterohaemorragic Escherichia coli. New Cells for New Vaccines: focus on plant and insect cells technologies, Coral Gables (Miami), FL, U.S.A. Ver resumen en PDF
Rigano MM, Alvarez ML, Pinkhasov J, Izzo AA, Cardi T, Walmsley AM (2006) The development of transgenic and transplastomic plants for production of a tuberculosis subunit vaccine. 50th Annual Congress of the Italian Society of Agriculture Genetics, Ischia (Napoles), ITALIA.
Alvarez ML, Pinyerd HL, Crisantes JD, Kilbourne J, Rigano MM, Pinkhasov J, Rocke T Cardineau GA (2006) Subunit vaccine developed in tomato targeted against plague and its immunogenicity in mice. Second Annual Vaccine Renaissance, Providence, RI, U.S.A. Ver resumen en PDF
Alvarez ML, Pinyerd HL, Crisantes JD, Kilbourne J, Rigano MM, Pinkhasov J, Cardineau GA Oral immunogenicity of an F1-V transgenic tomato vaccine targeted against plague (2006). 9th Annual Conference on Vaccine Research, Baltimore, MD, U.S.A. Ver resumen en PDF
Alvarez ML, Pinyerd HL, Crisantes JD, Pinkhasov J, Rigano MM, Kilbourne J, Mason HS Cardineau GA (2005) Plant-derived subunit vaccine for mucosal delivery targeted against pneumonic and bubonic plague. Meeting on Molecular and Immunological Approaches to Vaccine Design, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., U.S.A. Ver resumen en PDF
Pnkhasov J, Alvarez ML, Rigano MM, Mukherjee P, Gendler SJ, Arntzen CJ, Mason HS and Walmsley AM (2005). A MUC1-based plant engineered breast cancer vaccine. The fourth Era Hope meeting Department of Defense (DOD) Breast Cancer Research Program (BCRP), Philadelphia, Pennsylvania, U.S.A.
Alvarez ML, Pinyerd HL, Crisantes JD, Rigano MM, Pinkhasov J, Walmsley AM, Mason HS, Arntzen, Ch., Cardineau GA (2004). Stable plant expression of F1-V fusion protein antigens from Yersinia pestis for use as vaccine against plague REDBIO: V Latin American and Caribbean Meeting on Agriculture Biotechnology, Santo Domingo, REPUBLICA DOMINICANA. Ver resumen en PDF
Rigano MM, Alvarez ML, Pinkhasov J, Sala F, Arntzen CJ, Walmsley AM (2003). The use of transgenic plants for the production of vaccine proteins: an economical alternative ICABR’s 7th International Conference on: “Productivity, public Goods and Public Policy: Agricultural Biotechnology Potentials”, Ravello, ITALIA.
Rigano MM, Alvarez ML, Pinkhasov J, Sala F, Arntzen Ch, Walmsley AM (2003). Expression of a tuberculosis antigen in Arabidopsis thaliana and tomato plants” ISPMB (International Society of Plant Molecular Biology), 7th International Congress of Plant Molecular Biology, June 23-28, Barcelona, ESPAŇA.
Pinhasov J, Alvarez ML, Rigano MM, Mukherjee P, Gendler SJ, Arntzen CJ, Mason HS and Walmsley AM (2003). Development of a plant-derived breast cancer vaccine. ISPMB (International Society of Plant Molecular Biology), 7th International Congress of Plant Molecular Biology, Barcelona, ESPAŇA.
Alvarez ML, Gómez M, Carrillo JM, Guelman S, Halford N, Lustig S, Reggiardo M, Ryabushkina N, Shewry P, Stein J, Vallejos RH (2001) Obtención de trigo transgénico con mejor calidad panadera. V Congreso Nacional de Trigo y III Simposio Nacional de Cereales de Siembra Otoño Invernal, Villa Carlos Paz, ARGENTINA. Ver resumen en PDF
Alvarez ML, Guelman S, Halford NG, Lustig S, Reggiardo MI, Ryabushkina N, Shewry, P., Stein, J., Vallejos, R.H (1999). Gene silencing of endogenous high molecular weight glutenin subunits genes induced by the introduction of homologous transgenes in wheat IV Symposium in Plant Biotechnology. REDBIO, Buenos Aires, ARGENTINA. Ver resumen en PDF
Alvarez ML, Guelman S, Halford NG, Lustig S, Reggiardo MI, Ryabushkina N, Shewry P, Stein J, Vallejos RH (1999). Transgene-induced silencing of endogenous HMW glutenin subunits genes in wheat International Symposium in Common Themes in Transcription and RNA processing (ICGEB meeting), Buenos Aires, ARGENTINA. Ver resumen en PDF
Alvarez ML, Lustig S, Stein J, López M, Reggiardo M, Vallejos RH(1998). Silenciamiento de subunidades de glutenina de alto peso molecular endógenas por transformación con los genes de las subunidades 1Ax1 y 1Dx5" (1998). XXXIV Annual Meeting of Argentinean Society for the Research in Biochemistry and Molecular Biology (SAIB), Mendoza, ARGENTINA. Ver resumen en PDF
Vallejos RH, Alvarez ML, Halford NG, Heisterborg CM, Morata M, Ravizzini R, Shewry P (1996) Wheat genetic transformation as a tool contributing to breeding for quality. 10th International Cereal and Bread Congress, June 1996, Chalkidiki, GRECIA. Ver resumen en PDF
Vallejos RH, Alvarez ML, Bacigaluppo S, Halford NG, Heisterborg CM, Morata M, Ravizzini R, Shewry P (1996). Genetic engineering of wheat HMW glutenin for improving bread-making quality. VIII Congress of the Pan-American Association of Biochemistry and Molecular Biology societies (PABMB), Pucón, CHILE. Ver resumen en PDF
Vallejos RH, Alvarez ML, Cervigni G, Heisterborg CM, Morre J, Ortiz JP, Palena C, Permingeat H, Romagnoli MV, Rossi G, Zanor MI (1995) Genetic engineering of cereals. First International Symposium on novel and non-conventional plants: prospects for their practical use, Moscow, RUSIA. Ver resumen en PDF
Vallejos RH, Alvarez ML, Cervigni G¸ Heisterborg CM, Morre J, Morata M, Ortiz JP, Palena C, Permingeat H, Ravizzini R, Rossi G, Spitteler M (1995). Genetic engineering of cereals: Transgenic expression in maize and wheat. Biotecnología Habana ‘95: New Opportunities in Plant, Animal and Industrial Biotechnology, Havana, CUBA. Ver resumen en PDF
Rigano MM, Alvarez ML, Pinkhasov J, Izzo AA, Cardi T, Walmsley AM. (2007) Production of an oral, tuberculosis vaccine in transgenic and transplastomic plants. Plant transformation technologies. Vienna, February 4-7, AUSTRIA. Ver poster.
Alvarez ML, Pinyerd HL, Topal E, Ewing M, Crisantes JD, Cardineau GA (2007) Transient and stable expression of TBSV-P19 induces reversion of F1-V gene silencing in tomato. Gene Silencing: the Biology of Small RNAs and the Epigenome. 24th Symposium in Plant Biology. University of California, Riverside, CA, January 18-20, U.S.A. Ver resumen en PDF
Alvarez ML, Pinyerd HL, Crisantes JD, Kilbourne J, Rigano MM, Pinkhasov J, Rocke T, Cardineau GA (2006) Protection against plague in mice using an orally delivered F1-V transgenic tomato vaccine. New Cells for New Vaccines: focus on plant and insect cells technologies, Coral Gables (Miami), FL, September 28-29, U.S.A. Ver resumen en PDF
Topal E, Alvarez ML, O’Brien AD, Mason HS (2006) Plant-made intimin vaccine expressed in tomato for mucosal delivery to prevent colonization of enterohaemorragic Escherichia coli. New Cells for New Vaccines: focus on plant and insect cells technologies, Coral Gables (Miami), FL, U.S.A. Ver resumen en PDF
Rigano MM, Alvarez ML, Pinkhasov J, Izzo AA, Cardi T, Walmsley AM (2006) The development of transgenic and transplastomic plants for production of a tuberculosis subunit vaccine. 50th Annual Congress of the Italian Society of Agriculture Genetics, Ischia (Napoles), ITALIA.
Alvarez ML, Pinyerd HL, Crisantes JD, Kilbourne J, Rigano MM, Pinkhasov J, Rocke T Cardineau GA (2006) Subunit vaccine developed in tomato targeted against plague and its immunogenicity in mice. Second Annual Vaccine Renaissance, Providence, RI, U.S.A. Ver resumen en PDF
Alvarez ML, Pinyerd HL, Crisantes JD, Kilbourne J, Rigano MM, Pinkhasov J, Cardineau GA Oral immunogenicity of an F1-V transgenic tomato vaccine targeted against plague (2006). 9th Annual Conference on Vaccine Research, Baltimore, MD, U.S.A. Ver resumen en PDF
Alvarez ML, Pinyerd HL, Crisantes JD, Pinkhasov J, Rigano MM, Kilbourne J, Mason HS Cardineau GA (2005) Plant-derived subunit vaccine for mucosal delivery targeted against pneumonic and bubonic plague. Meeting on Molecular and Immunological Approaches to Vaccine Design, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., U.S.A. Ver resumen en PDF
Pnkhasov J, Alvarez ML, Rigano MM, Mukherjee P, Gendler SJ, Arntzen CJ, Mason HS and Walmsley AM (2005). A MUC1-based plant engineered breast cancer vaccine. The fourth Era Hope meeting Department of Defense (DOD) Breast Cancer Research Program (BCRP), Philadelphia, Pennsylvania, U.S.A.
Alvarez ML, Pinyerd HL, Crisantes JD, Rigano MM, Pinkhasov J, Walmsley AM, Mason HS, Arntzen, Ch., Cardineau GA (2004). Stable plant expression of F1-V fusion protein antigens from Yersinia pestis for use as vaccine against plague REDBIO: V Latin American and Caribbean Meeting on Agriculture Biotechnology, Santo Domingo, REPUBLICA DOMINICANA. Ver resumen en PDF
Rigano MM, Alvarez ML, Pinkhasov J, Sala F, Arntzen CJ, Walmsley AM (2003). The use of transgenic plants for the production of vaccine proteins: an economical alternative ICABR’s 7th International Conference on: “Productivity, public Goods and Public Policy: Agricultural Biotechnology Potentials”, Ravello, ITALIA.
Rigano MM, Alvarez ML, Pinkhasov J, Sala F, Arntzen Ch, Walmsley AM (2003). Expression of a tuberculosis antigen in Arabidopsis thaliana and tomato plants” ISPMB (International Society of Plant Molecular Biology), 7th International Congress of Plant Molecular Biology, June 23-28, Barcelona, ESPAŇA.
Pinhasov J, Alvarez ML, Rigano MM, Mukherjee P, Gendler SJ, Arntzen CJ, Mason HS and Walmsley AM (2003). Development of a plant-derived breast cancer vaccine. ISPMB (International Society of Plant Molecular Biology), 7th International Congress of Plant Molecular Biology, Barcelona, ESPAŇA.
Alvarez ML, Gómez M, Carrillo JM, Guelman S, Halford N, Lustig S, Reggiardo M, Ryabushkina N, Shewry P, Stein J, Vallejos RH (2001) Obtención de trigo transgénico con mejor calidad panadera. V Congreso Nacional de Trigo y III Simposio Nacional de Cereales de Siembra Otoño Invernal, Villa Carlos Paz, ARGENTINA. Ver resumen en PDF
Alvarez ML, Guelman S, Halford NG, Lustig S, Reggiardo MI, Ryabushkina N, Shewry, P., Stein, J., Vallejos, R.H (1999). Gene silencing of endogenous high molecular weight glutenin subunits genes induced by the introduction of homologous transgenes in wheat IV Symposium in Plant Biotechnology. REDBIO, Buenos Aires, ARGENTINA. Ver resumen en PDF
Alvarez ML, Guelman S, Halford NG, Lustig S, Reggiardo MI, Ryabushkina N, Shewry P, Stein J, Vallejos RH (1999). Transgene-induced silencing of endogenous HMW glutenin subunits genes in wheat International Symposium in Common Themes in Transcription and RNA processing (ICGEB meeting), Buenos Aires, ARGENTINA. Ver resumen en PDF
Alvarez ML, Lustig S, Stein J, López M, Reggiardo M, Vallejos RH(1998). Silenciamiento de subunidades de glutenina de alto peso molecular endógenas por transformación con los genes de las subunidades 1Ax1 y 1Dx5" (1998). XXXIV Annual Meeting of Argentinean Society for the Research in Biochemistry and Molecular Biology (SAIB), Mendoza, ARGENTINA. Ver resumen en PDF
Vallejos RH, Alvarez ML, Halford NG, Heisterborg CM, Morata M, Ravizzini R, Shewry P (1996) Wheat genetic transformation as a tool contributing to breeding for quality. 10th International Cereal and Bread Congress, June 1996, Chalkidiki, GRECIA. Ver resumen en PDF
Vallejos RH, Alvarez ML, Bacigaluppo S, Halford NG, Heisterborg CM, Morata M, Ravizzini R, Shewry P (1996). Genetic engineering of wheat HMW glutenin for improving bread-making quality. VIII Congress of the Pan-American Association of Biochemistry and Molecular Biology societies (PABMB), Pucón, CHILE. Ver resumen en PDF
Vallejos RH, Alvarez ML, Cervigni G, Heisterborg CM, Morre J, Ortiz JP, Palena C, Permingeat H, Romagnoli MV, Rossi G, Zanor MI (1995) Genetic engineering of cereals. First International Symposium on novel and non-conventional plants: prospects for their practical use, Moscow, RUSIA. Ver resumen en PDF
Vallejos RH, Alvarez ML, Cervigni G¸ Heisterborg CM, Morre J, Morata M, Ortiz JP, Palena C, Permingeat H, Ravizzini R, Rossi G, Spitteler M (1995). Genetic engineering of cereals: Transgenic expression in maize and wheat. Biotecnología Habana ‘95: New Opportunities in Plant, Animal and Industrial Biotechnology, Havana, CUBA. Ver resumen en PDF
Wednesday, October 10, 2007
LOGROS CIENTIFICOS MAS IMPORTANTES
Projecto: “Vacuna derivada de plantas para la prevención de peste bubónica y neumónica”
Lugar de trabajo: Centro de Enfermedades Infecciosas y Vacunas (CIDV)- Biodesign A - Arizona State University, Tempe, Arizona, U.S.A. (2003 – presente).
Cargo: Investigadora Post-doctoral
Lugar de trabajo: Centro de Enfermedades Infecciosas y Vacunas (CIDV)- Biodesign A - Arizona State University, Tempe, Arizona, U.S.A. (2003 – presente).
Cargo: Investigadora Post-doctoral
El objetivo de este proyecto fue expresar antígenos de la bacteria Yersinia pestis en tomate para desarrollar una vacuna oral derivada de plantas para prevenir peste bubónica y neumónica. Obtuvimos tomate transgénico expresando altos niveles de proteina de fusion F1-V (F1 y V son los dos antígenos más inmunogénicos de Y. pestis. El tomate conteniendo la vacuna fue disecado y convertido en polvo con el objeto de concentrar el antigeno F1-V y obtener una vacuna estable a temperatura ambiente. La vacuna derivada del tomate fue capaz de inducir una respuesta inmune satisfactoria en ratones. Los resultados obtenidos fueron publicados (Alvarez et al., 2004; Alvarez et al., 2006) y presentados en conferencias nacionales e internacionales.
La Dra. Alvarez diseñó y llevó a cabo todos los experimentos de este proyecto incluyendo la construcción de los plásmidos usados en la transformación, la selección y caracterización de las plantas transgénicas y los ensayos en animales para testear la vacuna derivada del tomate.
Projecto: “Reversión del silenciamiento génico de f1-v inducida por la expresión transiente y estable de TBSV-P19 en tomato”
Lugar de trabajo: Lugar de trabajo: Centro de Enfermedades Infecciosas y Vacunas (CIDV)- Biodesign A - Arizona State University, Tempe, Arizona, U.S.A. (2003- present).
La Dra. Alvarez diseñó y llevó a cabo todos los experimentos de este proyecto incluyendo la construcción de los plásmidos usados en la transformación, la selección y caracterización de las plantas transgénicas y los ensayos en animales para testear la vacuna derivada del tomate.
Projecto: “Reversión del silenciamiento génico de f1-v inducida por la expresión transiente y estable de TBSV-P19 en tomato”
Lugar de trabajo: Lugar de trabajo: Centro de Enfermedades Infecciosas y Vacunas (CIDV)- Biodesign A - Arizona State University, Tempe, Arizona, U.S.A. (2003- present).
Cargo: Investigadora Post-doctoral
El objetivo de este proyecto fue restaurar la expresión del gen f1-v en plantas de tomate silenciadas. A pesar de que estas plantas contenian un alto numero de copias del gen f1-v, los niveles de proteina F1-V fueron muy bajos o indetectables en hojas y frutos. Para recuperar la expresión del gen f1-v, super-transformamos las plantas silenciadas con el gen p19, un supresor de silenciamiento génico proveniente del virus del tomate TBSV. Las plantas que acumularon los mayores niveles de proteína P19 en transiente o en estable fueron también las que tuvieron el mayor contenido de proteina F1-V en hojas y frutos. Los niveles de proteinas F1-V en fruto de plantas con reversión de silenciamiento fueron significativamente mayores que en las plantas consideradas "F1-V elite" no silenciadas. Estos resultados confirman el potencial del uso de P19 para aumentar la acumulación de proteinas de interes en plantas transgenicas (Alvarez et al., manuscrito en revision enviado recientemente a la revista Plant Molecular Biology).
Dr. Alvarez diseñó y llevó a cabo todos los experimentos de este proyecto incluyendo la transformacion de tomate y la caracterizacion molecular de las plantas transgenicas obtenidas.
El objetivo de este proyecto fue restaurar la expresión del gen f1-v en plantas de tomate silenciadas. A pesar de que estas plantas contenian un alto numero de copias del gen f1-v, los niveles de proteina F1-V fueron muy bajos o indetectables en hojas y frutos. Para recuperar la expresión del gen f1-v, super-transformamos las plantas silenciadas con el gen p19, un supresor de silenciamiento génico proveniente del virus del tomate TBSV. Las plantas que acumularon los mayores niveles de proteína P19 en transiente o en estable fueron también las que tuvieron el mayor contenido de proteina F1-V en hojas y frutos. Los niveles de proteinas F1-V en fruto de plantas con reversión de silenciamiento fueron significativamente mayores que en las plantas consideradas "F1-V elite" no silenciadas. Estos resultados confirman el potencial del uso de P19 para aumentar la acumulación de proteinas de interes en plantas transgenicas (Alvarez et al., manuscrito en revision enviado recientemente a la revista Plant Molecular Biology).
Dr. Alvarez diseñó y llevó a cabo todos los experimentos de este proyecto incluyendo la transformacion de tomate y la caracterizacion molecular de las plantas transgenicas obtenidas.
Projecto: “Vacuna anticonceptiva derivada de plantas para uso en control de poblaciones de animales”
Lugar de trabajo: School of Life Sciences, Arizona State University, Tempe, Arizona, U.S.A. (2001 – 2002).
Cargos: Investigadora visitante y becaria de la Fundación Antorchas (Argentina).
The common brushtail possum is a marsupial native from Australia which was introduced to New Zealand by Europeans to establish a fur industry. They soon escaped into the wild where they have thrived as an invasive species with around 60 million individuals estimated. There have been numerous attempts to eradicate them because of the damage they do to native trees and wildlife, as well as acting as a carrier of bovine tuberculosis. The goal of this project was to develop an oral plant-derived vaccine to be used as immuno-contraceptive specie specific for possum population control. The seven amino acid epitope from ZP3 (zona pellucida glycoprotein 3) was fused to the heat-labile toxin (LT) of enterotoxigenic Escherichia coli and expressed in tomato. The fusion protein was found to assemble into pentamers and had an average expression level of 37.8 μg/g in freeze-dried transgenic tissues. The species-specific nature of this epitope was shown by the inability of antibodies raised against non-target species to detect the LTB fusion protein (Walmsley et al., 2003).
Dr Alvarez collaborated on this project doing the molecular characterization of the transgenic tomato plants expressing the fusion protein LTB-ZP3.
Project0: “Analisis de la funcionalidad de la masa derivada de harina de trigo transgénico”
Lugar de trabajo: Escuela Tecnica Superior de Ingenieros Agronomos (ETSIA), Departmento de Genetica y Mejoramiento de Plantas, Universidad Politecnica de Madrid (UPM), Madrid, ESPANA (2000)
Cargo: Research Scholar and Fellow of British Council and Fundacion Antorchas (Argentina).
The goal of this project was to determine the new physical properties of the dough made with flour from the different transgenic wheat lines expressing alleles of glutenins associated with better bread-making quality. The rheological properties of flours from five different lines of transgenic wheats that either express or over-express subunits 1Dx5 or 1Ax1 were analyzed by mixograph assays and SDS sedimentation tests. In one of the transgenic lines, the over-expression of subunit 1Dx5 resulted in a 2-fold increase in mixing time, associated with a significant improvement in dough strength, and a lower resistance breakdown, suggesting an important increase in dough quality (Alvarez et. al., 2001a; Alvarez et al., 2001 b).
Dr. Alvarez designed all the experiments and pursed all the dough analysis with the collaboration of colleagues at the Polytechnic University of Madrid, Spain.
Projecto (Tesis Doctoral): “Mejoramiento de la calidad nutricional y panadera del trigo por ingenieria genética".
Lugar de trabajo:
1) Centro de Estudios Fotosinteticos y Bioquimicos (CEFOBI), Universidad Nacional de Rosario, ARGENTINA (1995 – 2000)
2) Cell Biology Department, Institute of Arable Crops Research (IACR), Long Ashton Research Station, Bristol University, Bristol, INGLATERRA (2000)
Cargos:
1) Estudiante de doctorado y becaria del CONICET (Comision Nacional de Investigaciones Cientificas y Tecnicas) (1995-2000)
2) Cientifica visitante y becaria de British Council/ Fundación Antorchas (Argentina) (2000)
The main goal of this project was to improve the bread-making quality of wheat expressing high molecular weight (HMW) subunits of glutenins associated with better dough quality for bread.
Wheat HMW glutenin subunit genes 1Ax1 and 1Dx5 were introduced and either expressed or over-expressed into a commercial wheat cultivar that already expresses five subunits. Six independent transgenic wheat lines were obtained and characterized by SDS-PAGE and Southern-blot analyses. The 1Dx5 gene was over-expressed in two lines without changes in the other endosperm proteins. Two wheat lines expressed the 1Ax1 transgene with associated silencing of the 1Ax2* endogenous subunit. Southern analysis of the four events confirmed transformation and suggested that the transgenes were present in low gene copy number. Silencing of all the HMW glutenin subunits was observed in two different transgenic wheat lines expressing the 1Ax1 subunit transgene and over-expressing the 1Dx5 gene. Transgenes and expression patterns were stably transmitted to the progenies in all the events except one where in some of the segregating T2 seeds the silencing of all HMW glutenin subunits was reverted associated with a drastic loss of transgenes from a high to a low copy number.
This project was part of Dr. Alvarez's Ph.D Thesis and the results obtained were published in different journals (Alvarez et al., 2000; Alvarez et al., 2001).
Projecto: “Estudio de la presencia de fimbrias P en bacterias causantes de infección urinaria”.
Lugar de trabajo: Departmento de Microbiologia, Facultad de Bioquimica, Universidad Nacional de Rosario, ARGENTINA (1992-1993)
Cargo: Estudiante de Bioquimica y becaria de la fundacion de la Universidad Nacional de Rosario.
The long term goal of this project was to study the presence of glitter cells in the urinary sediment as a marker of high urinary infection by Enterobacterias mannose resistant that express fimbriae Pap. A total of 143 Enterobactereaceae strains isolated from patients with urinary infection were classified by biochemical and serological tests. The adhesive ability of the bacteria was determined using the human red cells group A agglutination test. The erythrocytes were suspended in phosphate buffer saline (PBS) either with or without mannose for testing mannose-resistant (MR-HA) or mannose- sensitive (MS-HA) hemagglutination. The number of Escherichia coli strains that expressed mannose-resistant fimbriae was 2-fold higher than the strains with mannose-sensitive fimbriae. There was a correlation between high urinary infections produced by E. coli strains with mannose-resistant fimbriae and presence of cells with special characteristics, glitter cells, in the urinary sediment.
Dr. Alvarez isolated and characterized the bacteria causing urinary infections using biochemical and serological tests. She determined the presence of mannose-resistant fimbriae in the bacteria and detected the glitter cells in the urinary sediment (Alvarez et al., 1994).
Lugar de trabajo: School of Life Sciences, Arizona State University, Tempe, Arizona, U.S.A. (2001 – 2002).
Cargos: Investigadora visitante y becaria de la Fundación Antorchas (Argentina).
The common brushtail possum is a marsupial native from Australia which was introduced to New Zealand by Europeans to establish a fur industry. They soon escaped into the wild where they have thrived as an invasive species with around 60 million individuals estimated. There have been numerous attempts to eradicate them because of the damage they do to native trees and wildlife, as well as acting as a carrier of bovine tuberculosis. The goal of this project was to develop an oral plant-derived vaccine to be used as immuno-contraceptive specie specific for possum population control. The seven amino acid epitope from ZP3 (zona pellucida glycoprotein 3) was fused to the heat-labile toxin (LT) of enterotoxigenic Escherichia coli and expressed in tomato. The fusion protein was found to assemble into pentamers and had an average expression level of 37.8 μg/g in freeze-dried transgenic tissues. The species-specific nature of this epitope was shown by the inability of antibodies raised against non-target species to detect the LTB fusion protein (Walmsley et al., 2003).
Dr Alvarez collaborated on this project doing the molecular characterization of the transgenic tomato plants expressing the fusion protein LTB-ZP3.
Project0: “Analisis de la funcionalidad de la masa derivada de harina de trigo transgénico”
Lugar de trabajo: Escuela Tecnica Superior de Ingenieros Agronomos (ETSIA), Departmento de Genetica y Mejoramiento de Plantas, Universidad Politecnica de Madrid (UPM), Madrid, ESPANA (2000)
Cargo: Research Scholar and Fellow of British Council and Fundacion Antorchas (Argentina).
The goal of this project was to determine the new physical properties of the dough made with flour from the different transgenic wheat lines expressing alleles of glutenins associated with better bread-making quality. The rheological properties of flours from five different lines of transgenic wheats that either express or over-express subunits 1Dx5 or 1Ax1 were analyzed by mixograph assays and SDS sedimentation tests. In one of the transgenic lines, the over-expression of subunit 1Dx5 resulted in a 2-fold increase in mixing time, associated with a significant improvement in dough strength, and a lower resistance breakdown, suggesting an important increase in dough quality (Alvarez et. al., 2001a; Alvarez et al., 2001 b).
Dr. Alvarez designed all the experiments and pursed all the dough analysis with the collaboration of colleagues at the Polytechnic University of Madrid, Spain.
Projecto (Tesis Doctoral): “Mejoramiento de la calidad nutricional y panadera del trigo por ingenieria genética".
Lugar de trabajo:
1) Centro de Estudios Fotosinteticos y Bioquimicos (CEFOBI), Universidad Nacional de Rosario, ARGENTINA (1995 – 2000)
2) Cell Biology Department, Institute of Arable Crops Research (IACR), Long Ashton Research Station, Bristol University, Bristol, INGLATERRA (2000)
Cargos:
1) Estudiante de doctorado y becaria del CONICET (Comision Nacional de Investigaciones Cientificas y Tecnicas) (1995-2000)
2) Cientifica visitante y becaria de British Council/ Fundación Antorchas (Argentina) (2000)
The main goal of this project was to improve the bread-making quality of wheat expressing high molecular weight (HMW) subunits of glutenins associated with better dough quality for bread.
Wheat HMW glutenin subunit genes 1Ax1 and 1Dx5 were introduced and either expressed or over-expressed into a commercial wheat cultivar that already expresses five subunits. Six independent transgenic wheat lines were obtained and characterized by SDS-PAGE and Southern-blot analyses. The 1Dx5 gene was over-expressed in two lines without changes in the other endosperm proteins. Two wheat lines expressed the 1Ax1 transgene with associated silencing of the 1Ax2* endogenous subunit. Southern analysis of the four events confirmed transformation and suggested that the transgenes were present in low gene copy number. Silencing of all the HMW glutenin subunits was observed in two different transgenic wheat lines expressing the 1Ax1 subunit transgene and over-expressing the 1Dx5 gene. Transgenes and expression patterns were stably transmitted to the progenies in all the events except one where in some of the segregating T2 seeds the silencing of all HMW glutenin subunits was reverted associated with a drastic loss of transgenes from a high to a low copy number.
This project was part of Dr. Alvarez's Ph.D Thesis and the results obtained were published in different journals (Alvarez et al., 2000; Alvarez et al., 2001).
Projecto: “Estudio de la presencia de fimbrias P en bacterias causantes de infección urinaria”.
Lugar de trabajo: Departmento de Microbiologia, Facultad de Bioquimica, Universidad Nacional de Rosario, ARGENTINA (1992-1993)
Cargo: Estudiante de Bioquimica y becaria de la fundacion de la Universidad Nacional de Rosario.
The long term goal of this project was to study the presence of glitter cells in the urinary sediment as a marker of high urinary infection by Enterobacterias mannose resistant that express fimbriae Pap. A total of 143 Enterobactereaceae strains isolated from patients with urinary infection were classified by biochemical and serological tests. The adhesive ability of the bacteria was determined using the human red cells group A agglutination test. The erythrocytes were suspended in phosphate buffer saline (PBS) either with or without mannose for testing mannose-resistant (MR-HA) or mannose- sensitive (MS-HA) hemagglutination. The number of Escherichia coli strains that expressed mannose-resistant fimbriae was 2-fold higher than the strains with mannose-sensitive fimbriae. There was a correlation between high urinary infections produced by E. coli strains with mannose-resistant fimbriae and presence of cells with special characteristics, glitter cells, in the urinary sediment.
Dr. Alvarez isolated and characterized the bacteria causing urinary infections using biochemical and serological tests. She determined the presence of mannose-resistant fimbriae in the bacteria and detected the glitter cells in the urinary sediment (Alvarez et al., 1994).
PROMOCION DE LOS RESULTADOS CIENTIFICOS OBTENIDOS
- Mejoramiento de la calidad del trigo por ingenieria genetica.
http://members.tripod.com/lucrecia_alvarez/espaniol.htm
- Vacunas derivadas de plantas
http://plant-madevaccinesespanol.blogspot.com/
En los periódicos:
- Diario Clarin (Buenos Aires, Argentina)
http://www.clarin.com/diario/2001/07/21/s-06801.htm
Felicitaciones de la Cámara de Diputados de la Nación Argentina:
http://www.diputados.gov.ar/dependencias/dcomisiones/periodo-118/118-3220.pdf
Páginas web:
http://members.tripod.com/lucrecia_alvarez/espaniol.htm
- Vacunas derivadas de plantas
http://plant-madevaccinesespanol.blogspot.com/
En los periódicos:
- Diario La Capital (Rosario, Argentina)
http://members.tripod.com/lucrecia_alvarez/la%20capital%201.htm
- Diario Clarin (Buenos Aires, Argentina)
http://www.clarin.com/diario/2001/07/21/s-06801.htm
Felicitaciones de la Cámara de Diputados de la Nación Argentina:
http://www.diputados.gov.ar/dependencias/dcomisiones/periodo-118/118-3220.pdf
Páginas web:
- Monsanto
http://www.monsanto.co.uk/news/ukshowlib.phtml?uid=10395
- Agrifood Awareness
http://www.afaa.com.au/news/n_news-1792.asp
- Asociacion de consumidores organicos
http://www.organicconsumers.org/ACO/articulos/article_5159.cfm
- Voz al mundo
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